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For cases where the LLM and the medical students differed, 2 senior annotators (board-certified Emergency Medicine physicians) adjudicated 126 discrepancies after standardizing the codebook and verifying IRR (Cohen k: eligibility=0.795, deprescribing=0.745). Notably, the confusion matrix (Figure 4) revealed that a major source of discrepancy was the significantly higher likelihood of the LLM to recommend deprescribing (11.6%) compared to the medical students (1.91%).
JMIR Aging 2025;8:e69504
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Peripheral blood mononuclear cell (PBMC) suspensions will be prepared within 24 hours of blood collection by low-density gradient centrifugation via Hermle Z300 (K#55085010) to avoid erythrophagy-related activation of the monocytes. PBMCs will be frozen and stored at –80 °C using 10% dimethylsulfoxide, thereby allowing all samples to be analyzed in parallel. Plasma will also be stored at –80 °C.
JMIR Res Protoc 2025;14:e66439
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