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Effect of a Web-Based Heartfulness Program on the Mental Well-Being, Biomarkers, and Gene Expression Profile of Health Care Students: Randomized Controlled Trial

Effect of a Web-Based Heartfulness Program on the Mental Well-Being, Biomarkers, and Gene Expression Profile of Health Care Students: Randomized Controlled Trial

The NEB Ultra II directional RNA-Seq Library Prep kit protocol was used to prepare libraries for messenger RNA (m RNA) sequencing (NEB, Cat# E7760 L). An initial concentration of 500 ng of the total RNA was taken for the assay. The m RNA molecules were captured using magnetic Poly(T) beads (NEB, Cat# E7490 L). Following purification, the enriched m RNA was fragmented using divalent cations under elevated temperatures.

Jayaram Thimmapuram, Kamlesh D Patel, Deepti Bhatt, Ajay Chauhan, Divya Madhusudhan, Kashyap K Bhatt, Snehal Deshpande, Urvi Budhbhatti, Chaitanya Joshi

JMIR Bioinform Biotech 2024;5:e65506

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Differences in Messenger RNA Expression of Fibulin-1, Elastin, Matrix Metalloproteinase-1, Basic Fibroblast Growth Factor, and α-Smooth Muscle Actin Between the Ventral and Dorsal Tunica Dartos in Patients With Hypospadias and Chordee: Protocol for a Prospective Cohort Study

Differences in Messenger RNA Expression of Fibulin-1, Elastin, Matrix Metalloproteinase-1, Basic Fibroblast Growth Factor, and α-Smooth Muscle Actin Between the Ventral and Dorsal Tunica Dartos in Patients With Hypospadias and Chordee: Protocol for a Prospective Cohort Study

Messenger RNA (m RNA) expression of these proteins may shed light on their roles in hypospadias [2,4,8]. However, research on dartos tunic elasticity, genetic factors, and m RNA expression in hypospadias is limited. A better understanding of the genes influencing dartos tunic elasticity could provide insights into pathophysiology and chordee management.

Joko Pitoyo, Alvarino, Eryati Darwin, Yanwirasti

JMIR Res Protoc 2024;13:e52282

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Differential Expression of Long Noncoding RNAs in Murine Myoblasts After Short Hairpin RNA-Mediated Dysferlin Silencing In Vitro: Microarray Profiling

Differential Expression of Long Noncoding RNAs in Murine Myoblasts After Short Hairpin RNA-Mediated Dysferlin Silencing In Vitro: Microarray Profiling

Lnc RNA and m RNA expression profiling were performed using Arraystar Mouse Lnc RNA Microarray V3.0 containing 21,486 lnc RNA and 18,921 m RNA probes. The lnc RNA probes were created based on the information derived from reputable transcriptome public databases, such as Refseq, UCSC known genes, and Ensembl and landmark publications. The percentage of probes made for each category of lnc RNAs is given in Table 1.

Richa Singhal, Rachel Lukose, Gwenyth Carr, Afsoon Moktar, Ana Lucia Gonzales-Urday, Eric C Rouchka, Bathri N Vajravelu

JMIR Bioinform Biotech 2022;3(1):e33186

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