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Recent studies have shown encouraging results including significant associations with serum calprotectin in patients with spondyloarthritis compared with matched controls and increased serum calprotectin levels correlated with disease activity in both rheumatoid arthritis and axial spondyloarthritis [11,12].
JMIR Res Protoc 2024;13:e62877
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We will aim not to exceed 24 hours and isolate serum within 1 or 2 hours of blood collection. Once the blood has been clotted, it will be centrifuged at 1000 x gravitational units (g) for 10 minutes in a refrigerated centrifuge (at 4˚C) to facilitate the separation of the serum. A barrier will form between the serum and the cells; if no barrier is formed, the sample will be respun for 5 minutes.
JMIR Res Protoc 2023;12:e48127
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We will obtain the serum from 5 m L of the blood sample of each participant and quantitatively measure serum SAA, Hcy, S100 B, BDNF levels. The blood samples will be obtained at baseline. Other blood measurements will be performed at the hospital’s laboratory as part of the visit, and values will be taken from the registry. All the necessary data that will be obtained from blood testing are mentioned in Table 14.
JMIR Res Protoc 2023;12:e50546
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Many previous studies investigating patient outcomes categorize sodium levels as hyponatremic (serum sodium concentration less than 135 m Eq/L), eunatremic, and hypernatremic (serum sodium concentration greater than 145 m Eq/L) [1,9-11]. Some studies also identified an increased risk of in-hospital and 1-year mortality in hospitalized patients with mild hyponatremia (125-134 m Eq/L) and hypernatremia (146-150 m Eq/L) [12,13].
JMIR Perioper Med 2023;6:e38462
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Based on the biomarkers reported in the literature, we chose to assess variations in the expression of 33 serum and urine biomarkers [9,28].
Biomarkers of urinary and intestinal microbiota may be prognostic of radiation-induced functional disorders [18-20]. Thus, we chose to assess changes in the composition of the urinary and fecal microbiota before, during, and after radiotherapy.
JMIR Res Protoc 2023;12:e38362
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