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Investigating the Effects of Hyperbaric Oxygen Treatment in Necrotizing Soft Tissue Infection With Transcriptomics and Machine Learning (the HBOmic Study): Protocol for a Prospective Cohort Study With Data Validation

Investigating the Effects of Hyperbaric Oxygen Treatment in Necrotizing Soft Tissue Infection With Transcriptomics and Machine Learning (the HBOmic Study): Protocol for a Prospective Cohort Study With Data Validation

Genes showing treatment-dependent differential regulation will be explored, as will genes involved in gene networks and signaling pathways related to, for example, host immunity, inflammation, and redox homeostasis. Gene set enrichment analyses will be performed with ranked log fold changes using state-of-the-art enrichment tools, such as the gse GO function in the cluster Profiler R package [38].

Julie Vinkel, Leonor Rib, Alfonso Buil, Morten Hedetoft, Ole Hyldegaard

JMIR Res Protoc 2022;11(11):e39252