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Spinal Cord Injury—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD (SATURN Study): Protocol of An Exploratory Study In Assessing the Safety and Efficacy of NeuroAiD Amongst People Who Sustain Severe Spinal Cord Injury

Spinal Cord Injury—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD (SATURN Study): Protocol of An Exploratory Study In Assessing the Safety and Efficacy of NeuroAiD Amongst People Who Sustain Severe Spinal Cord Injury

Spinal Cord Injury—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD (SATURN Study): Protocol of An Exploratory Study In Assessing the Safety and Efficacy of NeuroAiD Amongst People Who Sustain Severe Spinal Cord Injury

Authors of this article:

Ramesh Kumar1 Author Orcid Image ;   Ohnmar Htwe2 Author Orcid Image ;   Azmi Baharudin2 Author Orcid Image ;   Mohammad Hisam Ariffin2 Author Orcid Image ;   Shaharuddin Abdul Rhani2 Author Orcid Image ;   Kamalnizat Ibrahim2 Author Orcid Image ;   Aishah Rustam3 Author Orcid Image ;   Robert Gan3 Author Orcid Image
Article Authors Cited by (4) Tweetations (1) Metrics

    Original Paper

    1Department of Neurosurgery, Faculty of Medicine, University Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia

    2Department of Orthopaedics and Traumatology, Faculty of Medicine, University Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia

    3Medical Affairs, Moleac, Biopolis Way, Singapore

    Corresponding Author:

    Ramesh Kumar, FRCS (Neurosurgery)

    Department of Neurosurgery

    Faculty of Medicine

    University Kebangsaan Malaysia Medical Centre

    Jalan Yaacob Latif

    Kuala Lumpur,

    Malaysia

    Phone: 60 391455555

    Fax:60 391456684

    Email: rameshkumar71@hotmail.com


    Background: Spinal cord injury (SCI) is a devastating condition with limited therapeutic options despite decades of research. Current treatment options include use of steroids, surgery, and rehabilitation. Nevertheless, many patients with SCI remain disabled. MLC601 (NeuroAiD), a combination of natural products, has been shown to be safe and to aid neurological recovery after brain injuries and may have a potential role in improving recovery after SCI.

    Objective: The aim of this study is to evaluate the safety and efficacy of NeuroAiD amongst people who sustain SCI in the study setting.

    Methods: Spinal Cord Injury—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD (SATURN) is a prospective cohort study of patients with moderately severe to severe SCI, defined as American Spinal Injury Association (ASIA) Impairment Scale (AIS) A and B. These patients will be treated with open-label NeuroAiD for 6 months in addition to standard care and followed for 24 months. Anonymized data will be prospectively collected at baseline and months 1, 3, 6, 12, 18, and 24 and will include information on demographics; main diagnostics; and neurological and functional state assessed by the Spinal Cord Independence Measure, ASIA—International Standard for Neurological Classification Spinal Cord Injury, and Short Form (SF-8) Health Survey. In addition, NeuroAiD treatment, compliance, concomitant therapies, and side effects, if any, will be collected. Investigators will use a secured online system for data entry. The study is approved by the ethics committee of Hospital University Kebangsaan Malaysia.

    Results: The coprimary endpoints are safety, AIS grade, and improvement in ASIA motor score at 6 months. Secondary endpoints are AIS grade, ASIA motor scores and sensory scores, Spinal Cord Independence Measure (SCIM), SF-8 Health Survey, and compliance at other time points.

    Conclusions: SATURN investigates the promising role of NeuroAiD in SCI especially given its excellent safety profile. We described here the protocol and online data collection tool we will use for this prospective cohort study. The selection of moderately severe to severe SCI provides an opportunity to investigate the role of NeuroAiD in addition to standard rehabilitation in patients with poor prognosis. The results will provide important information on the feasibility of conducting larger controlled trials to improve long-term outcome of patients with SCI.

    Trial Registration: Clinicaltrials.gov NCT02537899; https://clinicaltrials.gov/ct2/show/NCT02537899 (Archived by WebCite at http://www.webcitation.org/6m2pncVTG)

    JMIR Res Protoc 2016;5(4):e230

    doi:10.2196/resprot.6275

    Keywords

    spinal cord injury; NeuroAiD; MLC601; MLC901; safety; recovery; efficacy 


    Background

    Spinal cord injury (SCI) is a devastating neurological disorder that affects thousands of individuals each year. Global incidence rate of traumatic SCI in 2007 was estimated at 23 cases per million (133,000 to 226,000 cases per annum) and prevalence was between 236 to 4187 per million [1].

    Over the past decades, much progress has been made in our understanding of the molecular and cellular events in SCI, providing insights into important mechanisms of tissue damage and failure of regeneration of injured neurons. Current treatment options for SCI include the use of high-dose methylprednisolone and surgical interventions to stabilize and decompress the spinal cord in the acute period, while rehabilitation is provided as long-term management. There is currently no treatment that enhances recovery after the injury, and SCI remains to be a devastating condition for which therapeutic options are still limited [2].

    Three decades of clinical research on interventions to improve neurological outcomes in persons with SCI has not translated the promise of preclinical discovery into a consensus standard of care treatment. Nonetheless, SCI researchers remain hopeful that advances in preclinical discovery coupled with improved clinical trial performance will yield effective restorative treatment [3].

    There have been many lessons learned from past failures in clinical trials, including patient selection based on knowledge of prognosis for spontaneous natural recovery and other eligibility criteria, clinical trial design, and outcome measures [4-7]. By taking the lessons into consideration in selecting a series of cases to be treated, important insights may be gained into the potential role of NeuroAiD as a therapy for SCI.

    MLC601 (NeuroAiD, Nu-rAiD) is a combination of 14 natural ingredients indicated as treatment for poststroke recovery widely used in China and in many countries in Asia [8,9]. In Europe, a simplified formulation of the product, MLC901 (NeuroAid II, NurAiD II), consisting of 9 herbal components is available and will soon be available in Asian countries as well. Both formulations shall collectively be referred to as “NeuroAiD” in this study.

    NeuroAiD efficacy and safety are supported by preclinical and clinical studies. The neuroprotective and neuroproliferative properties of NeuroAiD have been extensively elucidated during in vitro and in vivo experiments using animal and cellular models of focal and global ischemia [8-11]. In addition, research on its positive effects in traumatic brain injury (TBI) has recently been published [12,13]. What are remarkable are the effects of NeuroAiD on neurogenesis and neurorestoration beyond mere neuroprotection.

    Case series reports of the use of NeuroAiD in neurosurgical conditions have been the subject of publications and presentations in international neurosurgical congresses [14-16]. In addition, there are several ongoing studies on the use of NeuroAiD in poststroke cognitive impairment and TBI [17-19].

    The clinical data on NeuroAiD, however, are most well-reported in stroke. A systematic review of randomized clinical trials on NeuroAiD showed its benefits in improving functional outcomes and neurological deficits with 3 months treatment among patients with ischemic stroke in the preceding 1 week to 6 months [20-25]. Subsequently, NeuroAiD was investigated in acute ischemic stroke within 72 hours of onset which demonstrated the treatment effect to be larger in postacute and relatively more severe stroke at 3 months [26-29]. The updated meta-analysis showed a pooled odds ratio in favor of NeuroAiD [27]. Furthermore, treatment with NeuroAiD was associated with a reduction in risk of early vascular events after a stroke [30]. More recently, the extension study of this randomized, placebo-controlled, double blind trial has provided evidence of its benefit on long-term functional outcome persisting over time up to 18 months after a stroke with an excellent safety profile [31].

    Since 2001 when it was marketed in China, there have been minimal serious side effects reported to date with the use of NeuroAiD. The common side effects reported from NeuroAiD were mostly mild and transient. Excellent clinical safety has been demonstrated in published clinical trials which reported the more common adverse events being gastrointestinal (nausea, vomiting, discomfort, diarrhea, dry mouth) and headache [20-34]. Safety studies in humans have shown that NeuroAiD, given alone or combined with aspirin, had no effect on clotting and coagulation [35]. Furthermore, there was no effect on hematological, hemostatic, and biochemical parameters or electrocardiogram in normal and stroke patients, even when started within 48 hours of stroke onset [35-37].

    Study Objectives

    The primary objective of this cases series is to evaluate the safety and potential role of NeuroAiD in SCI.


    Study Design and Subject Eligibility

    Spinal Cord Injury—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD (SATURN) is a prospective cohort study of patients with moderately severe to severe SCI treated with open-label NeuroAiD in addition to standard care (Figure 1).

    As this is an open-label study, inclusion of patients with SCI who are likely to spontaneously recover may confound the results of the study. Potential prognostic factors are severity of SCI and time from injury to assessment [4]. Therefore, patients are included in the study if they meet all of the following inclusion and none of the exclusion criteria (see Textbox 1).

    ‎
    Figure 1. Spinal Cord Injury—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD (SATURN) study flowchart.
    View this figure
    Selection criteria for the study.

    Inclusion criteria:

    • Male or female
    • Age 18 to 65 years
    • Diagnosed with SCI between 3 days and 4 weeks
    • American Spinal Injury Association (ASIA) Impairment Scale (AIS) A or B
    • Informed consent obtained

    Exclusion criteria:

    • Nonsurvivable injury
    • Multiple significant trauma (ie, significant intracranial and extracranial injuries including limb fractures) that would limit observation of recovery from spinal cord injury
    • Other conditions that would limit clinical assessment of outcomes (eg, dementia, demyelinating disease, autoimmune disease)
    • Refusal of treatment or contraindication to NeuroAiD
    Textbox 1. Selection criteria for the study.

    Study Setting and Recruitment of Participants

    Information about the study will be disseminated to the hospital and department staff. Any potential subject referred to the study team will be prescreened for potential eligibility. Permission to approach the patient or their legal representative will be obtained from the primary physician. Participants who fulfil the eligibility criteria will be recruited while still in the hospital for treatment or rehabilitation within the specified time window from injury. Informed consent will be obtained from all participants after discussion of the nature, purpose, and potential risks of the study.

    Treatment

    Each 400 mg capsule of MLC601 contains 9 herbal ingredients (extracts of Radix astragali, Radix salviae miltiorrhizae, Radix paeoniae rubra, Rhizoma chuanxiong, Radix angelicae sinensis, Carthamus tinctorius, Prunus persica, Radix polygalae, and Rhizoma acori tatarinowii) and 5 nonherbal components (Hirudo, Eupolyphaga seu steleophaga, Calculus bovisartifactus, Buthus martensii, and Cornu saigae tataricae). MLC901 contains only the 9 herbal extracts.

    The product is available in capsule form and administered orally or the contents may be diluted in water and administrated via gastric tube. The dosage is 4 capsules 3 times a day for MLC601 and 2 capsules 3 times a day for MLC901. The treatment duration is 6 months.

    The capsules should be kept sealed until opened for administration and stored below 30°C in a dry place. NeuroAiD is manufactured according to applicable control measures that ensure the consistency and quality of the product from batch to batch and adhere to good manufacturing practice. The active ingredients and finished product are subjected to full quality control testing for safety.

    All participants are allowed to receive standard care and other therapies and treatments including, but not limited to, surgery, rehabilitation, and other types of care deemed appropriate and as prescribed by their physician. There is no restriction to the use of any other treatment as recommended by the treating physician although other treatments should be recorded in the database.

    Variables Collected

    Subjects will undergo assessments at baseline and at months 1 (±7 days), 3 (±14 days), 6 (±14 days), 12 (±30 days), 18 (±30 days), and 24 (±30 days) (see Table 1). Information collected is meant to specifically address the objectives of the study.

    Table 1. Schedule of Spinal Cord Injury—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD (SATURN) study procedures.


    Information collected    		Base-lineMonth 1
    ±7d    		Month 3
    ±14d    		Month 6
    ±14d    		Month 12
    ±30d    		Month 18
    ±30d    		Month 24
    ±30d
    DemographicsX





    Diagnosis and medical conditionX





    Surgical historyX





    AISa gradeXXXXXXX
    ASIA ISNCSCIb worksheetXXXXXXX
    NeuroAiD treatment/complianceXXXX


    SCIMc
    		XXXXXX
    SF-8d Health SurveyXXXXXXX
    Concomitant treatments/therapiesXXXXXXX
    Adverse eventsXXXXXXX

    aAIS: American Spinal Injury Association Impairment Scale.

    bASIA ISNCSCI: American Spinal Injury Association International Standard for Neurological Classification Spinal Cord Injury.

    cSCIM: Spinal Cord Independence Measure.

    dSF: Short Form.

    Data collected at baseline immediately prior to or at the start of NeuroAiD treatment will include

    • Demographics data: date of birth, gender, ethnicity
    • Details of diagnosis of SCI: date of occurrence; spinal cord level; cause of spinal cord injury; magnetic resonance imaging, computed tomography, or x-ray results; presence of specific sequelae of SCI (eg, respiratory failure, pneumonia, circulatory problems, spasticity and muscle tone, autonomic dysreflexia, pain, bladder and bowel dysfunctions, sexual dysfunction)
    • AIS grade (pre- and postsurgery if patient undergoes surgery)
    • ASIA International Standard for Neurological Classification Spinal Cord Injury (ISNCSCI) worksheet (pre- and postsurgery if patient undergoes surgery)
      • Motor subscores for each limb and total motor score based on ASIA
      • Sensory subscores based on ASIA
      • Other items in the ASIA ISNCSCI worksheet
    • NeuroAiD use: date started and dose
    • Concomitant medications and treatments including any surgical intervention (date performed), treatments administered (start date and stop date), and rehabilitation (ie, physical and occupational therapies [start date, stop date, location—rehabilitation center, home-based]).

    Data collected from month 1 to month 24 will include compliance with intake of NeuroAiD, occurrence of any adverse event, Spinal Cord Independence Measure (SCIM), and Short Form (SF)-8 Health Survey, in addition to the other clinical assessments performed at baseline.

    Data Collection

    The investigators or designated personnel must record all required participant data in their entirety to ensure accurate interpretation of data. An explanation must be documented for any missing data. Data will be collected through an online data entry system [38] which is compliant with the Health Information Privacy and Security Act. Contributors to the study will be provided secured access accounts with username and password. Paper report forms are available if online submission is not possible (eg, Internet downtime, computer malfunction, power outage) but online entry is the preferred mode of data collection. If paper forms are used, data must be written in a neat and legible manner using black or blue ballpoint pen to ensure the clarity of the reproduced copy of all completed forms which are signed and dated. The completed online or paper forms shall serve as the source documents. No other medical record or source document will be required for this study.

    Study Endpoints

    The primary endpoints for this study are the AIS grade at 6 months, the improvement in ASIA total motor score at 6 months compared to baseline, and safety.

    The secondary endpoint measures will be the neurological recovery of the subjects as assessed by

    • AIS grade at 1, 3, 12, 18, and 24 months
    • ASIA motor scores at 1, 3, 12, 18, and 24 months
    • ASIA sensory scores at 1, 3, 6, 12, 18, and 24 months
    • SCIM at 1, 3, 6, 12, 18, and 24 months
    • SF-8 Health Survey at 1, 3, 6, 12, 18, and 24 months
    • Compliance to NeuroAiD at 1, 3, and 6 months

    Safety Considerations

    Definition of Adverse Event and Serious Adverse Event

    An adverse event is defined as any untoward medical occurrence in a person administered a product which does not necessarily have a causal relationship with this treatment. An adverse event is considered a serious adverse event if it results in death, persistent or significant disability, abortion, congenital anomaly, or birth defect; is life-threatening; or requires inpatient hospitalization or prolongation of existing hospitalization.

    Side Effect (or Adverse Drug Reaction)

    A side effect is an effect, whether therapeutic or adverse, that is secondary to the one intended. It can also apply to beneficial, but unintended, consequences of the use of a treatment. For the purpose of this study, a side effect (or adverse drug reaction) is any unintended adverse event that is related to the use of the treatment, NeuroAiD. Based on causality as defined by the World Health Organization–Uppsala Monitoring System [39], any adverse event that is considered by the treating physician as being possibly, probably, or definitely related to NeuroAiD would be considered as a side effect.

    Reporting of Adverse Events and Side Effects

    All adverse events or laboratory abnormalities that develop during the course of the participant’s treatment will have to be reported in the study. Adverse events should be reported as a diagnosis or syndrome. If this is not possible, the specific symptom or abnormality may be entered. The start date of first onset of any sign or symptom of the event and stop date (date the event is considered to have resolved, if resolved) should be entered. Seriousness should be classified according to the definition of a serious adverse event.

    Side effects that are reported in the study, especially those categorized as serious, will trigger a request for more medical information to ascertain details and need for reporting as serious adverse reaction to local regulatory authorities, if required.

    Follow-Up of Adverse Events

    Any adverse event must be followed until resolution, the condition stabilizes, the event is explained, or the participant is lost to follow-up. The physician is responsible to ensure that standard medical diagnostic or therapeutic management, if any, is performed.

    Common Side Effects Reported From NeuroAiD

    The common side effects reported from NeuroAiD use were mostly mild and transient. These include dry mouth, nausea, vomiting, abdominal discomfort, diarrhea, and headache. In many cases, the side effect resolved with reduction of the dose by half for one week, resumed at the usual dose after resolution of the symptom.

    Ethical Considerations

    Approval from the ethics committee of the Hospital University Kebangsaan Malaysia has been obtained for this study. Patient information sheet and informed consent form as approved by the ethics committee will be used to explain to the subjects the nature and purpose of their participation prior to performing any study-related procedures.

    The potential risks involved in participating in the study will be explained to the patient. The medical judgement regarding the use of NeuroAiD as treatment for SCI is a joint decision of the physician and the patient or legal representative. This decision must be arrived at prior to and without consideration of the patient’s potential participation in the study registry. Possible common side effects of the treatment and in very rare cases severe allergic reaction or unexpected life-threatening events will be explained.

    Statistical Consideration

    The target number of participants for this exploratory cohort study is set at 30. Descriptive statistics will be used to summarize data. Outcome assessments will be compared to baseline and previous observations. Comparison with appropriate historical controls with the same AIS grading and timeframe will be performed [4]. Multiple variable analyses will be used to identify predictors of better outcome, when appropriate. Other analyses deemed appropriate by the investigators and statistical consultant will also be performed.

    Study Administration and Oversight

    The study shall be carried out in the Hospital University Kebangsaan Malaysia, Kuala Lumpur, Malaysia, led by the principal investigator (RK) and assisted by coinvestigators (OH, AB, MHA, SAR, KI).

    Data Management

    The online data entry system is compliant with the Health Information Privacy and Security Act. Data will be maintained in a secured database in Moleac (Singapore) accessible only to relevant personnel. Subjects’ identities will be recorded only as initials with identification numbers. Specific individual information in the study will not be shared with other persons, entities, or companies unless required by legal authorities. Collective anonymized information will be summarized and reviewed. These summaries may be presented to stakeholders (eg, physicians, regulatory authorities, attendees in a conference) and/or published in scientific journals.

    Adherence and Amendments to Protocol

    Study investigators must adhere to the protocol and ensure that it is strictly followed. If necessary, an amendment may be implemented only after approval is obtained for the amendment from the ethics committee, except where necessary to eliminate an immediate hazard to participants or when changes involve any logistical or administrative aspects of the study (eg, change of personnel, change of telephone number). Amendments may not remove any of the basic data elements as described in this protocol.

    Closure of Study

    The study shall be closed when the number of study participants has been reached and the investigators agree that further inclusion of participants is no longer relevant or necessary. The closure will occur in steps, starting with informing investigators of the plan to discontinue inclusion of more participants and until after the follow-up of the last participant has been completed. The database will subsequently be locked upon an agreed timeframe with the principal investigator.


    The study is currently recruiting patients and is expected to complete in June 2018.


    The treatment options for SCI are limited. The reasons for the failure of earlier clinical trials to find new therapies in SCI have been extensively discussed [3-7]. Exploring new therapeutic strategies from natural substances is attractive and has gained much attention recently. The SATURN study investigates the promising role of NeuroAiD in SCI using an open-label cohort study design which is appropriate for a first-ever safety and efficacy study of a new treatment in this condition. Nevertheless, the design of the study takes much of past learnings into consideration. SATURN would include more severe and less acute patients among whom true treatment effect may be better demonstrated by reducing spontaneous recovery as a confounder. Neurological, functional, and quality of life outcomes are measured. As recovery and neuroplasticity may take time to accrue, the period of treatment and observation is extended to detect any delayed benefits which may not be apparent during the first few months of SCI. Published cohorts of patients with the same severity and timeframe may serve as historical controls. The knowledge gained from SATURN will certainly provide important insights on safety and efficacy in planning future clinical trials.

    Acknowledgments

    Saiful Najaib (Moleac Singapore) programmed and maintains the online data collection system for the study.

    Authors' Contributions

    RK is the principal investigator of this study. RK, OH, AB, MHA, SAR, KI, AR, and RG have contributed substantially to the conceptualization and design of the study. RK, OH, AB, MHA, and SAR will have equal contributions in patient recruitment, treatment, follow-up, data analysis, and writing of the manuscript reporting the results of the study.

    Conflicts of Interest

    This study is supported in part by Moleac Singapore Pte Ltd, 11 Biopolis Way #09-08 Helios, Singapore 138667. RK has received support for clinical trials and presentations at scientific meetings from Moleac Singapore. AR and RG are employees of Moleac Singapore. Other than support for this study, the other authors have no other competing interests to declare.

    1. Lee BB, Cripps RA, Fitzharris M, Wing PC. The global map for traumatic spinal cord injury epidemiology: update 2011, global incidence rate. Spinal Cord 2014 Feb;52(2):110-116. [CrossRef] [Medline]
    2. Silva NA, Sousa N, Reis RL, Salgado AJ. From basics to clinical: a comprehensive review on spinal cord injury. Prog Neurobiol 2014 Mar;114:25-57. [CrossRef] [Medline]
    3. Lammertse DP. Clinical trials in spinal cord injury: lessons learned on the path to translation. The 2011 International Spinal Cord Society Sir Ludwig Guttmann Lecture. Spinal Cord 2013 Jan;51(1):2-9. [CrossRef] [Medline]
    4. Fawcett JW, Curt A, Steeves JD, Coleman WP, Tuszynski MH, Lammertse D, et al. Guidelines for the conduct of clinical trials for spinal cord injury as developed by the ICCP panel: spontaneous recovery after spinal cord injury and statistical power needed for therapeutic clinical trials. Spinal Cord 2007 Mar;45(3):190-205. [CrossRef] [Medline]
    5. Steeves JD, Lammertse D, Curt A, Fawcett JW, Tuszynski MH, Ditunno JF, et al. Guidelines for the conduct of clinical trials for spinal cord injury (SCI) as developed by the ICCP panel: clinical trial outcome measures. Spinal Cord 2007 Mar;45(3):206-221. [CrossRef] [Medline]
    6. Tuszynski MH, Steeves JD, Fawcett JW, Lammertse D, Kalichman M, Rask C, et al. Guidelines for the conduct of clinical trials for spinal cord injury as developed by the ICCP Panel: clinical trial inclusion/exclusion criteria and ethics. Spinal Cord 2007 Mar;45(3):222-231. [CrossRef] [Medline]
    7. Lammertse D, Tuszynski MH, Steeves JD, Curt A, Fawcett JW, Rask C, et al. Guidelines for the conduct of clinical trials for spinal cord injury as developed by the ICCP panel: clinical trial design. Spinal Cord 2007 Mar;45(3):232-242 [FREE Full text] [CrossRef] [Medline]
    8. Heurteaux C, Gandin C, Borsotto M, Widmann C, Brau F, Lhuillier M, et al. Neuroprotective and neuroproliferative activities of NeuroAid (MLC601, MLC901), a Chinese medicine, in vitro and in vivo. Neuropharmacology 2010 Jun;58(7):987-1001. [CrossRef] [Medline]
    9. Quintard H, Borsotto M, Veyssiere J, Gandin C, Labbal F, Widmann C, et al. MLC901, a traditional Chinese medicine, protects the brain against global ischemia. Neuropharmacology 2011 Sep;61(4):622-631. [CrossRef] [Medline]
    10. Moha Ou Maati H, Borsotto M, Chatelain F, Widmann C, Lazdunski M, Heurteaux C. Activation of ATP-sensitive potassium channels as an element of the neuroprotective effects of the traditional Chinese medicine MLC901 against oxygen glucose deprivation. Neuropharmacology 2012 Sep;63(4):692-700. [CrossRef] [Medline]
    11. Heurteaux C, Widmann C, Moha ou Maati H, Quintard H, Gandin C, Borsotto M, et al. NeuroAiD: properties for neuroprotection and neurorepair. Cerebrovasc Dis 2013;35 Suppl 1:1-7 [FREE Full text] [CrossRef] [Medline]
    12. Quintard H, Lorivel T, Gandin C, Lazdunski M, Heurteaux C. MLC901, a traditional Chinese medicine induces neuroprotective and neuroregenerative benefits after traumatic brain injury in rats. Neuroscience 2014 Sep 26;277:72-86 [FREE Full text] [CrossRef] [Medline]
    13. Tsai M, Chang C, Peng S, Jhuang K, Fang Y, Lin M, et al. Therapeutic efficacy of Neuro AiD (MLC 601), a traditional Chinese medicine, in experimental traumatic brain injury. J Neuroimmune Pharmacol 2015 Mar;10(1):45-54. [CrossRef] [Medline]
    14. Yeo T, Chou N. Case report on the use of MLC601 (NeuroAiD) in neurosurgical pathologies. In: Int J Stroke. 2010 Presented at: World Stroke Congress; 2010; Seoul, South Korea p. 308.
    15. Kumar R, Fadzil F, Soon B. Feasibility of using NeuroAiD in neurological pathologies in NeST registry online. Presented at: International Conference of Neurology and Epidemiology; 2014; Kuala Lumpur, Malaysia.
    16. Kumar R, Fadzil F, Soon B. MLC601 (NeuroAiD) for brain injuries: open label use in a series of patients. Presented at: Asian Australasian Congress of Neurological Surgeons; 2015; Jeju, South Korea.
    17. Chen CL, Ikram K, Anqi Q, Yin WT, Chen A, Venketasubramanian N. The NeuroAiD II (MLC901) in vascular cognitive impairment study (NEURITES). Cerebrovasc Dis 2013;35 Suppl 1:23-29 [FREE Full text] [CrossRef] [Medline]
    18. Navarro JC, Chen CL, Lagamayo PD, Geslani MB, Eow GB, Poungvarin N, et al. CHIMES-I: sub-group analysis of the effects of NeuroAiD according to baseline brain imaging characteristics among patients randomized in the CHIMES study. Int J Stroke 2013 Aug;8(6):491-494. [CrossRef] [Medline]
    19. Australian New Zealand Clinical Trials Registry. Brain injury and NeuroAiD supplementation.   URL: https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=364449 [accessed 2016-06-20] [WebCite Cache]
    20. Siddiqui FJ, Venketasubramanian N, Chan ES, Chen C. Efficacy and safety of MLC601 (NeuroAiD), a traditional Chinese medicine, in poststroke recovery: a systematic review. Cerebrovasc Dis 2013;35 Suppl 1:8-17 [FREE Full text] [CrossRef] [Medline]
    21. Chen C, Venketasubramanian N, Gan RN, Lambert C, Picard D, Chan BP, et al. Danqi Piantang Jiaonang (DJ), a traditional Chinese medicine, in poststroke recovery. Stroke 2009 Mar;40(3):859-863 [FREE Full text] [CrossRef] [Medline]
    22. Harandi A, Abolfazli R, Hatemian A, Ghragozlee K, Ghaffar-Pour M, Karimi M, et al. Safety and efficacy of MLC601 in Iranian patients after stroke: a double-blind, placebo-controlled clinical trial. Stroke Res Treat 2011;2011 [FREE Full text] [CrossRef] [Medline]
    23. Ghandehari K, Mood Z, Ebrahimzadeh S, Picard D, Zhang Y. NeuroAiD (MLC601) versus piracetam in the recovery of postinfarct homonymous hemianopsia. Neural Regen Res 2011;6(6):418-422. [CrossRef]
    24. Bavarsad SR, Shamsaei G, Pakdaman H, Majdinasab N, Nejad EM, Sajedi SA, et al. The effect of NeuroAiD (MLC601) on cerebral blood flow velocity in subjects' post brain infarct in the middle cerebral artery territory. Eur J Intern Med 2011 Oct;22(5):509-513. [CrossRef] [Medline]
    25. Kong KH, Wee SK, Ng CY, Chua K, Chan KF, Venketasubramanian N, et al. A double-blind, placebo-controlled, randomized phase II pilot study to investigate the potential efficacy of the traditional Chinese medicine Neuroaid (MLC 601) in enhancing recovery after stroke (TIERS). Cerebrovasc Dis 2009;28(5):514-521. [CrossRef] [Medline]
    26. Venketasubramanian N, Chen CL, Gan RN, Chan BP, Chang HM, Tan SB, et al. A double-blind, placebo-controlled, randomized, multicenter study to investigate Chinese Medicine Neuroaid Efficacy on Stroke recovery (CHIMES Study). Int J Stroke 2009 Feb;4(1):54-60. [CrossRef] [Medline]
    27. Chen CL, Young SH, Gan HH, Singh R, Lao AY, Baroque AC, et al. Chinese medicine neuroaid efficacy on stroke recovery: a double-blind, placebo-controlled, randomized study. Stroke 2013 Aug;44(8):2093-2100 [FREE Full text] [CrossRef] [Medline]
    28. Navarro JC, Gan HH, Lao AY, Baroque AC, Hiyadan JH, Chua CL, et al. Baseline characteristics and treatment response of patients from the Philippines in the CHIMES study. Int J Stroke 2014 Oct;9 Suppl A100:102-105 [FREE Full text] [CrossRef] [Medline]
    29. Chankrachang S, Navarro JC, de Silva DA, Towanabut S, Chua CL, Lee CF, et al. Prognostic factors and treatment effect in the CHIMES study. J Stroke Cerebrovasc Dis 2015 Apr;24(4):823-827 [FREE Full text] [CrossRef] [Medline]
    30. Chen CL, Venketasubramanian N, Lee CF, Wong KS, Bousser M. Effects of MLC601 on early vascular events in patients after stroke: the CHIMES study. Stroke 2013 Dec;44(12):3580-3583 [FREE Full text] [CrossRef] [Medline]
    31. Venketasubramanian N, Young SH, Tay SS, Umapathi T, Lao AY, Gan HH, et al. Chinese medicine NeuroAiD efficacy on stroke recovery—extension study (CHIMES-E): a multicenter study of long-term efficacy. Cerebrovasc Dis 2015;39(5-6):309-318 [FREE Full text] [CrossRef] [Medline]
    32. Navarro JC, Molina MC, Baroque II AC, Lokin JK. The use of NeuroAiD (MLC601) in postischemic stroke patients. Rehabil Res Pract 2012 [FREE Full text] [CrossRef] [Medline]
    33. Siow CH. NeuroAiD in stroke recovery. Eur Neurol 2008;60(5):264-266 [FREE Full text] [CrossRef] [Medline]
    34. Kaishibayev N, Khassenova G, Kaishibayeva G. Efficacy of NeuroAiD II in the treatment of stroke patients. Presented at: World Stroke Congress; 2012; Brasilia, Brazil.
    35. Gan R, Lambert C, Lianting J, Chan ES, Venketasubramanian N, Chen C, et al. Danqi Piantan Jiaonang does not modify hemostasis, hematology, and biochemistry in normal subjects and stroke patients. Cerebrovasc Dis 2008;25(5):450-456. [CrossRef] [Medline]
    36. Young SH, Zhao Y, Koh A, Singh R, Chan BP, Chang H, et al. Safety profile of MLC601 (Neuroaid) in acute ischemic stroke patients: a Singaporean substudy of the Chinese medicine NeuroAiD efficacy on stroke recovery study. Cerebrovasc Dis 2010;30(1):1-6. [CrossRef] [Medline]
    37. Bavarsad SR, Hemati A, Hosseinzadeh MA. A randomized trial to assess the long-term safety of NeuroAiD among Caucasian patients with acute ischemic stroke. Chin J Integr Med 2014 Nov;20(11):812-817. [CrossRef] [Medline]
    38. SATURN—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD.   URL: https://www.neuroaid.com/en/saturn/ [accessed 2016-11-16] [WebCite Cache]
    39. World Health Organization. NeuroAiD. The use of the WHO-UMC system for standardized case causality assessment.   URL: http://www.who-umc.org/Graphics/24734.pdf [accessed 2016-11-15] [WebCite Cache]

    ‎
    AIS: American Spinal Injury Association Impairment Scale
    ASIA: American Spinal Injury Association
    ISNCSCI: International Standard for Neurological Classification Spinal Cord Injury
    SATURN: Spinal Cord Injury—Assessing Tolerability and Use of Combined Rehabilitation and NeuroAiD
    SCI: spinal cord injury
    SCIM: Spinal Cord Independence Measure
    SF-8: Short Form 8-Item Health Survey
    TBI: traumatic brain injury

    Edited by G Eysenbach; submitted 27.06.16; peer-reviewed by CLH Chen, N Pefile; comments to author 04.10.16; revised version received 06.11.16; accepted 08.11.16; published 05.12.16

    Copyright

    ©Ramesh Kumar, Ohnmar Htwe, Azmi Baharudin, Mohammad Hisam Ariffin, Shaharuddin Abdul Rhani, Kamalnizat Ibrahim, Aishah Rustam, Robert Gan. Originally published in JMIR Research Protocols (http://www.researchprotocols.org), 05.12.2016.

    This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Research Protocols, is properly cited. The complete bibliographic information, a link to the original publication on http://www.researchprotocols.org, as well as this copyright and license information must be included.